P786

PALEČEK, FRANTIŠEK. Measurement of ventilatory mechanics in the rat. J. Appl. Physiol. 27(1): 149-156. 1969.-- A method for repeated measurements of the following ventilatory parameters in the rat has been developed: tidal volume, frequency, functional residual lung capacity, and lung resistance and compliance. Construction of a body plethysmograph for the rat is described. A technic of tracheal intubation of the rat is demonstrated. The signal of volume changes from the plethysmograph are transformed by electronic derivation to provide the airflow signal. Intrathoracic pressure changes are recorded from a water-filled tube located in the esophagus. These three variables provide information on lung resistance and compliance. The functional residual lung capacity is ascertained manometrically from a simultaneous record of volume and intratracheal pressure changes during inspiratory efforts of the animal into a closed system. The whole examination can be performed in inhalation (halothane) anesthesia and repeated as necessary during the animal's life. Results of repeated measurements in a group of rats are presented.